PokéBase - Pokémon Q&A
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I never evolved any of them before. So does it
- remain with you in the bag, or
- is the evolved Pokemon holding it, or
- does it disappear forever?

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It will disappear forever, just like using an Evolutionary Stone

Source: Experience playing the game.

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5th of November, your username is my birthday. :D
My nans birthday is the 5th november lol
:o o wow